• AAV Biosensors


    Vigene's AAV Biosensor products come as ready-to-use AAV vectors. The vectors are genetically encoded with your chosen biosensor, either calcium or glutamate sensors. These products are ready for in vivo injection. These biosensors are made possible through a world-wide license from Janelia Research Campus, HHMI.

    Light up nerve cells as they communicate

    Explore biosensors range

    View biosensors gallery

    Learn more: What are biosensors

    How to select Vigene biosensors?

    Download Vigene's AAV-biosensor product handbook

    AAV Biosensors Gallery

    Images showcased here are from Vigene customers. Where applicable, investigators have provided links to their lab page and contact details. Click on an image below to view larger.

    Biosensor Range

    Our current available biosensor range includes a range of calcium and glutamate biosensors:

    CaMPARI
    higher-throughput Ca assays
    GCaMP3
    Modified GCaMP
    GCaMP5
    Modified GCaMP
    GCaMP6
    Modified GCaMP
    jRCaMP1
    Modified GCaMP
    jRGECO1
    Modified GCaMP
      iGLuSnFR
    Glutamate sensors
     

    We are updating this product listing continuously. We have more exciting products in the pipeline, so watch this space. Learn How do you choose an AAV biosensor.

    Key AAV Biosensor Benefits

    ● Genetically encoded biosensors packaged in AAV from $499

    ● Monitor and measure fluctuations in molecular signals in cells

    ● Monitor calcium and glutamate

    ● Labelling of “active” cells within tissues

    ● Useful for multifunctional imaging for drug screening

    ● Fully customizable (additional charges may apply)

    What are Biosensors?

    Biosensors are genetically engineered fluorescent proteins (FP) attached to an additional protein sequence which makes them sensitive to small biomolecules or other physiological intracellular processes (e.g. Ca2+). These biosensors are introduced into cells, tissues or organisms to detect changes by fluorescence microscopy intensity or spectrum change. Many biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, biosensors permit signaling pathway exploration or allow for the measurement of a biomolecule. They do all this while principally preserving both spatial as well as temporal cellular processes.


    Fluorescent Protein Biosensors diagram - How they work

    Schematic representation of FP-based biosensor. The fusion of an FP such as GFP (green) to a specific binding domain, shown in gray, can be used to report on the production of certain signaling molecules. Ca2+ sensors consists of a molecular switch that contains calmodulin (CaM) and M13 inserted into a circularly permuted GFP, shown in green, in which the native N- and C-termini of GFP are linked together, and new termini are generated from within the core Β-barrel structure of GFP; the addition of Ca2+ causes CaM to bind to M13, which leads to increased GFP fluorescence.


    How do you choose an AAV biosensor?

    ● Decide which biosensor is most suitable for your research question - calcium or glutamate; for example for GCaMP6, decide whether you need GCaMP s or m, f.

    ● Decide whether you would like to include FLEX Learn more.

    ● Choose a promoter (universal promoter - CAG, or tissue specificneuron specific-synapsin promoter).

    ● Choose the AAV vector serotype (AAV1-9; AAV8 and AAV9 are ready-to-ship).

    What to do after receiving your biosensors

    All AAV-biosensors come in ready-to-inject and ready-to-transduce format of AAV vectors. Upon receipt, please store them in -80C.

    Have other questions?

    Please contact us. We are more than happy to answer any of your viral vector questions.


    Legal Statement of AAV Biosensor

    1. AAV Biosensors are covered under US Patents #14/350,199; #8,629,256, #14/800,814, #14/800,814, #14/941,406, #14/974,483, 14/941,406, 14/974,483, 14/452,428 and foreign equivalents and licensed from Janelia Research Campus, HHMI, Janelia, Virginia, USA.

    2. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude any human or clinical use.

    3. The purchase of the AAV Biosensor Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from Vigene Biosciences Inc. services and any related material solely for Research Purposes only, not for any Commercial Purposes.