• AAV Biosensors


    Vigene's AAV biosensors come ready-to-use and our product range includes a variety of calcium and glutamate biosensors made possible through a world-wide license from Janelia Research Campus, HHMI. Please see our AAV Biosensors Handbook for more information.

    What are Biosensors?

    Biosensors are genetically engineered fluorescent proteins (FP) attached to an additional protein sequence which makes them sensitive to small biomolecules or other physiological intracellular processes (e.g. Ca2+). These biosensors are introduced into cells, tissues or organisms to detect changes by fluorescence microscopy intensity or spectrum change. Many biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, biosensors permit signaling pathway exploration or allow for the measurement of a biomolecule. They do all this while principally preserving both spatial as well as temporal cellular processes.


    Fluorescent Protein Biosensors diagram - How they work

    Schematic representation of FP-based biosensor. The fusion of an FP such as GFP (green) to a specific binding domain, shown in gray, can be used to report on the production of certain signaling molecules. Ca2+ sensors consists of a molecular switch that contains calmodulin (CaM) and M13 inserted into a circularly permuted GFP, shown in green, in which the native N- and C-termini of GFP are linked together, and new termini are generated from within the core Β-barrel structure of GFP; the addition of Ca2+ causes CaM to bind to M13, which leads to increased GFP fluorescence.

    Biosensor Range

    Our currently available biosensor range includes a range of calcium and glutamate biosensors. Please click on any of the below to see more product information.

    CaMPARI
    higher-throughput Ca assays
    GCaMP3
    Modified GCaMP
    GCaMP5
    Modified GCaMP
    GCaMP6
    Modified GCaMP
    jRCaMP1
    Modified GCaMP
    jRGECO1
    Modified GCaMP
      iGLuSnFR
    Glutamate sensors
     

    We have more exciting biosensor products in our pipeline, so we will be updating this product listing continuously. Please check back periodically or contact us if you have a specific request.

    Key AAV Biosensor Benefits

    ● Genetically encoded biosensors packaged in AAV from $499

    ● Monitor and measure fluctuations in molecular signals in cells

    ● Monitor calcium and glutamate

    ● Labelling of “active” cells within tissues

    ● Useful for multifunctional imaging for drug screening

    ● Fully customizable (additional charges may apply)

    Have questions or want to request a quote?

    Please contact us!

    AAV Biosensors Gallery

    Images showcased here are a Vigene customer. Click on the below images to enlarge.


    Legal Statement

    1. AAV Biosensors are covered under US Patents #14/350,199; #8,629,256, #14/800,814, #14/800,814, #14/941,406, #14/974,483, 14/941,406, 14/974,483, 14/452,428 and foreign equivalents and licensed from Janelia Research Campus, HHMI, Janelia, Virginia, USA.

    2. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude any human or clinical use.

    3. The purchase of the AAV Biosensor Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from Vigene Biosciences Inc. services and any related material solely for Research Purposes only, not for any Commercial Purposes.