• AAV Biosensors


    Vigene's AAV Biosensor products come as ready-to-use AAV vectors. The vectors are genetically encoded with your chosen biosensor, either calcium or glutamate sensors. These products are ready for in vivo injection. These biosensors are made possible through a world-wide license from Janelia Research Campus, HHMI.

    Our current available biosensor range includes a range of calcium and glutamate biosensors: CaMPARI, GCaMP3, GCaMP5, GCaMP6,jRCaMP1, jRGECO1 , iGLuSnFR . See full list of AAV Biosensors. We are updating this product listing continuously. We have more exciting products in the pipeline, so watch this space. Learn How do you choose an AAV biosensor.

    What are Biosensors?

    Biosensors are genetically engineered fluorescent proteins (FP) attached to an additional protein sequence which makes them sensitive to small biomolecules or other physiological intracellular processes (e.g. Ca2+). These biosensors are introduced into cells, tissues or organisms to detect changes by fluorescence microscopy intensity or spectrum change. Many biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, biosensors permit signaling pathway exploration or allow for the measurement of a biomolecule. They do all this while principally preserving both spatial as well as temporal cellular processes.


    Fluorescent Protein Biosensors diagram - How they work

    Schematic representation of FP-based biosensor. The fusion of an FP such as GFP (green) to a specific binding domain, shown in gray, can be used to report on the production of certain signaling molecules. Ca2+ sensors consists of a molecular switch that contains calmodulin (CaM) and M13 inserted into a circularly permuted GFP, shown in green, in which the native N- and C-termini of GFP are linked together, and new termini are generated from within the core Β-barrel structure of GFP; the addition of Ca2+ causes CaM to bind to M13, which leads to increased GFP fluorescence.
    Table. AAV Biosensors

    Click on the biosensor name to find out more information. Biosensors are provided as 100µl volume, titer at 1013GC/ml

    NameBiosensorPromoterDescriptionProduct Name
    CaMPARI CalciumSynhigher-throughput calcium assays with cultured cellspAAV-synapsin-CaMPARI
      Syn pAAV-synapsin-FLEX-CaMPARI
      CAG pAAV-CAG-FLEX-CaMPARI
    GCaMP6s Calcium indicatorSynimproved SNR, slower kinetics;Green indicatorpGP-AAV-syn-GCaMP6s-WPRE.4.641
      Syn pGP-AAV-syn-flex-GCaMP6s-WPRE.24.641
      CAG pGP-AAV-CAG-flex-GCaMP6s-WPRE.25.641
    GCaMP6m CalciumSynimproved SNR, intermediate kinetics;Green indicatorpGP-AAV-syn-GCaMP6m-WPRE.4.629
      Syn pGP-AAV-syn-flex-GCaMP6m-WPRE.24.629
      CAG pGP-AAV-CAG-flex-GCaMP6m-WPRE.25.629
    GCaMP6f CalciumSynimproved SNR, faster kinetics; Green indicatorpGP-AAV-syn-GCaMP6f-WPRE.4.693
      Syn pGP-AAV-syn-flex-GCaMP6f-WPRE.24.693
      CAG pGP-AAV-CAG-flex-GCaMP6f-WPRE.25.693
    GCaMP3 CalciumCAGGreen calcium indicatorpGP-AAV_CAG-GCaMP3
    GCaMP5 Calcium Green calcium indicatorpRSET.GCaMP5G(7.35)
    jRCaMP1a CalciumSynred, improved SNR, slower kinetics, brightpGP-AAV-syn-NES-jRCaMP1a-WPRE.211.1488
      Syn pGP-AAV-syn-flex-NES-jRCaMP1a-WPRE.215.1488
      CAG pGP-AAV-CAG-flex-NES-jRCaMP1a-WPRE.216.1488
    jRCaMP1b CalciumSynred, improved SNR, improved dynamic range, brightpGP-AAV-syn-NES-jRCaMP1b-WPRE.211.1519
      Syn pGP-AAV-syn-flex-NES-jRCaMP1b-WPRE.215.1519
      CAG pGP-AAV-CAG-flex-NES-jRCaMP1b-WPRE.216.1519
    jRGECO1a CalciumSynred, improved SNRpGP-AAV-syn-NES-jRGECO1a-WPRE.111.1670
      Syn pGP-AAV-syn-flex-NES-jRGECO1a-WPRE.115.1670
      CAG pGP-AAV-CAG-flex-NES-jRGECO1a-WPRE.116.1670
    jRGECO1b CalciumSynred, improved SNRpGP-AAV-syn-NES-jRGECO1b-WPRE.111.1721
      Syn pGP-AAV-syn-flex-NES-jRGECO1b-WPRE.115.1721
      CAG pGP-AAV-CAG-flex-NES-jRGECO1b-WPRE.116.1721
    iGLuSnFR GlutamateCMVRapid detection of glutamate, improved SNR; direct visualization of synaptic release (as opposed to Ca2+ imaging)YbeJ253.L1LVL2NP.pMinDis

    How do you choose an AAV biosensor?

    1 - Decide which biosensor is most suitable for your research question - calcium or glutamate; for example for GCaMP6, decide whether you need GCaMP s or m, f.
    2 - Decide whether you would like to include FLEX Learn more.
    3 - Choose a promoter (universal promoter - CAG, or tissue specificneuron specific-synapsin promoter).
    4 - Choose the AAV vector serotype (AAV1-9; AAV8 and AAV9 are ready-to-ship).

     

    What to do after receiving your biosensors

    All AAV-biosensors come in ready-to-inject and ready-to-transduce format of AAV vectors. upon receipt, please store them in -80C. For detailed cell and in vivo applications, please refer to our AAV-biosensor product handbook.


    Have other questions? Please contact us. We are more than happy to answer any of your viral vector questions.


    Legal Statement of AAV Biosensor

    1. AAV Biosensors are covered under US Patents #14/350,199; #8,629,256, #14/800,814, #14/800,814, #14/941,406, #14/974,483, 14/941,406, 14/974,483, 14/452,428 and foreign equivalents and licensed from Janelia Research Campus, HHMI, Janelia, Virginia, USA.

    2. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude any human or clinical use.

    3. The purchase of the AAV Biosensor Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from Vigene Biosciences Inc. services and any related material solely for Research Purposes only, not for any Commercial Purposes.