• AAV Biosensors - CaMPARI Calcium indicator


    Vigene's CaMPARI products come ready-to-use, with a choice of promoter and the ability to include the Cre inducible (FLEx-ON) expression. We have packaged CaMPARI into the most commonly used AAV serotypes (AAV8 and AAV9). Should you require a different serotype, please contact us.

    CaMPARI: A Photoconvertible Protein for Calcium Activity History Imaging

    ● CaMPARI, or Calcium Modulated Photoactivatable Ratiometric Integrator, is a photoconvertible
    protein construct, enabling imaging of integrated calcium activity of large populations of cells
    over defined time windows.

    ● Calcium, as an important regulator of many cellular signaling events, and calcium indicators have found extensive use for imaging and measuring changes in Ca2+ levels associated with neural activity.

    CaMPARI is based on EosFP [PubMed], a fluorescent protein whose emission changes from green to red upon irradiation with UV-light (~400 nm). By engineering libraries of EosFP variants, inventors were able to develop a protein that undergoes significantly faster green-to-red photoconversion (PC) only when calcium is present while PC light is applied. This permanent conversion provides the ability to record calcium activity for all areas illuminated by PC-light. The red fluorescence intensity correlates with calcium activity. CaMPARI complements existing calcium indicators by allowing total calcium activity measurement over large areas of cells and tissues.

    CaMPARI Biosensors

    Biosensors are provided as 100 µl volume, titer at 1013GC/ml


    NamePromoterProduct NameAAVCat #Price
    CaMPARISynpAAV-synapsin-CaMPARIAAV8BS10-NORAAV8 $499
       AAV9BS10-NORAAV9 $499
     SynpAAV-synapsin-FLEX-CaMPARIAAV8BS10-NXRAAV8 $499
       AAV9BS10-NXRAAV9 $499
     CAGpAAV-CAG-FLEX-CaMPARIAAV8BS10-CXRAAV8 $499
       AAV9BS10-CXRAAV9 $499

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    Key advantage of CaMPARI

    ● Image total calcium activity during defined time windows (gated by photoconversion light).
    ● Not restricted to the field of view of a microscope, as during real-time calcium imaging with e.g. GCaMP.
    ● Enables higher-throughput calcium assays with cultured cells.
    ● Calcium activity imaging of across large cell populations and/or tissues.
    ● Labeling of “active” cells within a tissue (such as brain) during stimulus or behavior in model organisms.
    ● Tracing of neurons based on their calcium activity level.

    References

    1. Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER Science. 2015 Feb 13;347(6223):755-60. doi: 10.1126/science.1260922.