• AAV Biosensors - GCaMP Calcium indicators


    Vigene offers GCaMP3, GCaMP5 and GCaMP6 products come ready-to-use, with a choice of promoter with or without Cre inducible (FLEx-ON) expression. AAV8 and AAV9 serotypes of AAV-GCaMP6 are ready to ship. Should you require a different serotype, please contact us.

    GCaMP: Next-generation genetically encoded calcium indicators

    ● GCaMP is a genetically encoded calcium indicator (GECI) generated from a fusion of the green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. Upon binding of GECI to Ca2+ there is an induction of a change in fluorescence signal. This signal change allows measurement of action potentials, and other receptor activation events, that trigger Ca2+ fluxes.

    ● Applications include neuroscience research using in vitro or in vivo models; long-term functional imaging of neuronal activity including correlation with animal behavior; screening for G-protein coupled receptor (GPCR) or ion channel agonists and antagonists.

    GCaMP Biosensors

    Biosensors are provided as 100 µl volume, titer at 1013GC/ml


    NamePromoterProduct NameAAVCat #Price
    GCaMP6sSynpGP-AAV-syn-GCaMP6s-WPRE.4.641AAV8BS1-NOSAAV8 $499
       AAV9BS1-NOSAAV9 $499
     SynpGP-AAV-syn-flex-GCaMP6s-WPRE.24.641AAV8BS1-NXSAAV8 $499
       AAV9BS1-NXSAAV9 $499
     CAGpGP-AAV-CAG-flex-GCaMP6s-WPRE.25.641AAV8BS1-CXSAAV8 $499
       AAV9BS1-CXSAAV9 $499
    GCaMP6mSynpGP-AAV-syn-GCaMP6m-WPRE.4.629AAV8BS2-NOMAAV8 $499
       AAV9BS2-NOMAAV9 $499
     SynpGP-AAV-syn-flex-GCaMP6m-WPRE.24.629AAV8BS2-NXMAAV8 $499
       AAV9BS2-NXMAAV9 $499
     CAGpGP-AAV-CAG-flex-GCaMP6m-WPRE.25.629AAV8BS2-CXMAAV8 $499
       AAV9BS2-CXMAAV9 $499
    GCaMP6fSynpGP-AAV-syn-GCaMP6f-WPRE.4.693AAV8BS3-NOFAAV8 $499
       AAV9BS3-NOFAAV9 $499
     SynpGP-AAV-syn-flex-GCaMP6f-WPRE.24.693AAV8BS3-NXFAAV8 $499
       AAV9BS3-NXFAAV9 $499
     CAGpGP-AAV-CAG-flex-GCaMP6f-WPRE.25.693AAV8BS3-CXFAAV8 $499
       AAV9BS3-CXFAAV9 $499
    GCaMP3 CAG AAV_CAG-GCaMP3 AAV8 BS4-CX3AAV8 $499
       AAV9BS4-CX3AAV9 $499
    GCaMP5G pRSET.GCaMP5G(7.35)AAV8BS5-PXAAAV8 $499
       AAV9BS5-PXAAAV9 $499

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    Key advantage of GCaMP

    ● Genetically specified for Ready for use studies in vivo.
    ● GCaMP6 are the 6th generation of GCaMP, offering improved engineering for increased signal-to-noise ratio and much faster kinetics, when compared to previous versions, specifically, GCaMP3 and GCaMP5G.
    ● GCaMP3 is the 3rd generation of GCaMP and reliably detects three or more action potentials in short bursts in several systems in vivo.

    References

    1. Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354