• AAV Biosensors - iGluSnFR Glutamate indicator


    Vigene's iGluSnFR products come ready-to-use, with a choice of promoter and the ability to include the Cre inducible (FLEx-ON) expression. We have packaged iGluSnFR into the most commonly used AAV serotypes (AAV8 and AAV9). Should you require a different serotype, please contact us.

    iGluSnFR: An Optimized Intensity-Based Sensing Fluorescent Reporter

    ● Glutamate, an important signaling molecule, is the major neurotransmitter in the brain, playing a critical role in nearly all aspects of normal brain function. Dysregulation in glutamate is associated with stroke and neurodegenerative disorders including Alzheimer's disease. As such an important molecule it is no wonder scientists, especially neuroscientists, find glutamate valuable enough to want to visualize as well as quantitate it.

    ● Optimized single wavelength glutamate sensor engineered for maximum fluorescence response and validated in complex in vivo systems.

    ● Applications include use in Neurobiology research using in vitro or in vivo models, including long-term imaging studies, as well as glutamate signaling research in non-neuronal tissues.

    ● Delivers improved means to directly map excitatory synaptic activity in the brain.

    Glutamate Biosensors

    Biosensors are provided as 100 µl volume, titer at 1013GC/ml


    NamePromoter Product Name AAV Cat # Price
    iGluSnFRCMV pCMV(MinDis).iGluSnFR AAV8 BS11-COG-AAV8 $499
          AAV9 BS11-COG-AAV9 $499

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    Key advantage of iGluSnFR

    ● Extremely rapid glutamate detection with high spatial resolution.

    ● Improved signal-to-noise ratio compared to existing fluorescent glutamate biosensors.

    ● Genetically-encoded, thereby can be targeted to specific cellular populations and sub-cellular locations.

    ● Enables direct visualization of synaptic release (as opposed to Ca2+ imaging).

    ● Long-term in vivo imaging of glutamate signaling in worms, zebrafish, and mice.

    References

    1. Marvin JS, Borghuis BG, Tian L, et al. An optimized fluorescent probe for visualizing glutamate neurotransmission. Nature methods. 2013;10(2):162-170. doi:10.1038/nmeth.2333.