• Delivery with Precision - ORF Shuttling Systems


    To better accommodate the diverse need of tagging and transferring Human ORFs with different tag types and/or different locations Vigene Biosciences has pioneered the pEnter Entry Vector that is compatible with a variety of destinations vectors.

    A large panel of destination vectors are available so that you can express the ORF with different tags, with tags at different ends of the protein, or as the native, untagged protein.

    The ORF of an entry clone can be shuttled into all destination vectors and other viral vectors through a simple "cut and paste" restriction enzyme digestion and ligation. A combination of two pairs of restriction enzymes, AsiSI/MluI (96%), AsiSI/RsrII(99%) covers 99.5% of all human cDNA ORFs. All human genes can be covered with a few additional restriction enzyme combinations, such as AsiSI/NotI, AscI/RsrII.

    Vigene offers a range of vector products, with all vectors shipping next day as lyophilized 5 µg DNA.

    Shuttling Vectors

    Adenoviral Shuttling Vectors

    Lentiviral Shuttling Vectors

    AAV Shuttling Vectors

    shRNA Shuttling Vectors

    Key Advantages

    More than 30 destination vectors available, including different C & N-terminal affinity tags and GFP & RFP fluorescent tags. Learn mroe about tags.

    Accommodate large inserts up to 30kb: Entry Vector designed to accommodate large inserts up to 30kb & very toxic genes without the need for a special E.coli strain.

    Easy shuttling with a simple cut-and-paste approach.

    ORF transfer can be completed in 2-3 hours, more cost-effective and efficient comapred to Gateway Systems.

    ● Both Entry vector and destination vectors are designed for adenovirus production.

    adenovirus construction schematic


    Schematic representation of an ORF shuttling system designed for adenovirus production. All of the destination vectors can be recombined with engineered adenoviral vectors through homologous recombination in E. coli.

     

    Adenoviral Shuttling Vectors

    All vectors ship NEXT DAY as lyophilized 5 µg DNA.

    • Cat.#
    • Vector
    • Selection
    • Promoter
    • Tag
    • Tag Position
    • Price
    P100001 pENTER Kanamycin CMV Flag, His C-terminal
    $249
    P100002 pDEST-C-GFP Ampicillin CMV GFP C-terminal
    $249
    P100003 pDEST-C-RFP Ampicillin CMV RFP C-terminal
    $249
    P100004 pDEST-C-HA Ampicillin CMV HA C-terminal
    $249
    P100005 pDEST-C-3XFlag Ampicillin CMV 3XFlag C-terminal
    $249
    P100006 pDEST-C-Myc Ampicillin CMV Myc C-terminal
    $249
    P100008 pDEST-N-His Ampicillin CMV His N-terminal
    $249
    P100009 pDEST-N-Flag Ampicillin CMV Flag N-terminal
    $249
    P100010 pDEST-N-Myc Ampicillin CMV Myc N-terminal
    $249
    P100011 pDEST-N-HA Ampicillin CMV HA N-terminal
    $249
    P100012 pDEST-N-GFP Ampicillin CMV GFP N-terminal
    $249
    P100013 pDEST-N-RFP Ampicillin CMV RFP N-terminal
    $249
    P100014 pDEST-EF1a Ampicillin EF1a Flag, His C-terminal
    $249
    P100015 pDEST-PGK Ampicillin PGK Flag, His C-terminal
    $249


    Lentiviral Vectors

    All vectors ship NEXT DAY as lyophilized 5 µg DNA.

    • Cat.#
    • Vector
    • Selection
    • Promoter
    • Tag
    • Tag Position
    • Price
    P100020 pLent-Flag-His Ampicillin CMV Flag-His ORF
    $249
    P100021 pLent-GFP-CMV Ampicillin CMV Myc-Flag ORF
    $249
    P100022 pLent-Puro-CMC Ampicillin CMV Myc-Flag ORF
    $249
    P100023 pLent-C-GFP Ampicillin CMV GFP ORF
    $249
    P100024 pLent-C-RFP Ampicillin CMV RFP ORF
    $249
    P100025 pLent-N-GFP Ampicillin CMV GFP ORF
    $249
    P100026 pLent-N-RFP Ampicillin CMV RFP ORF
    $249
    P100027 pLent-N-Flag Ampicillin CMV Flag ORF
    $249
    P100028 pLent-N-Myc Ampicillin CMV Myc ORF
    $249
    P100029 pLent-N-HA Ampicillin CMV HA ORF
    $249


    AAV Vectors

    All vectors ship NEXT DAY as lyophilized 5 µg DNA.

    • Cat.#
    • Vector
    • Selection
    • Promoter
    • Tag
    • Tag Position
    • Price
    P100034 pAV-FH Ampicillin CMV Flag-His ORF
    $249


    shRNA Vectors

    All vectors ship NEXT DAY as lyophilized 5 µg DNA.

    • Cat.#
    • Vector
    • Selection
    • Promoter
    • Tag
    • Tag Position
    • Price
    P100016 pSH-H1-GFP Kanamycin H1 GFP Adenovirus
    $249
    P100017 pSH-H1-RFP Kanamycin H1 RFP Adenovirus
    $249
    P100018 pSH-U6-GFP Kanamycin U6 GFP Adenovirus
    $249
    P100019 pSH-U6-RFP Kanamycin U6 RFP Adenovirus
    $249
    P100030 pLent-H1-GFP Ampicillin H1 N/A Lentivirus
    $249
    P100031 pLent-H1-RFP Ampicillin H1 N/A Lentivirus
    $249
    P100032 pLent-U6-GFP Ampicillin U6 N/A Lentivirus
    $249
    P100033 pLent-U6-RFP Ampicillin U6 N/A Lentivirus
    $249
    P100035 pAV-H1-GFP Ampicillin H1 GFP AAV
    $249
    P100036 pAV-H1-RFP Ampicillin H1 RFP AAV
    $249
    P100037 pAV-U6-GFP Ampicillin U6 GFP AAV
    $249
    P100038 pAV-U6-RFP Ampicillin U6 RFP AAV
    $249