• Lentiviral particles of Full-length Human cDNA


    Lentiviral vectors are gene delivery tools derived from a modified from of HIV-1; most of the viral genes have been removed. Vigene offers an extensive range of human cDNA open reading frames (ORFs) cloned into a lentiviral expression vector and can be used to overexpress human genes and proteins in mammalian cells.

    Vigene features the complete human cDNA genome in ready-to-use lentiviral particle formats. Offering lentiviral particles of high-quality and high-titer. Additionally, if desired titer is not achieved we will re-package in an additional run, free of charge. Save your research efforts in preparing your own virus, and receive ready-to-transfect lentiviral particles.

    Lentiviral cDNA ORFs

    Search Lentivirus by name, gene or accession

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    Quality Virus Packaging Service Guarantees

    ● Competitive pricing, varies by gene.

    ● Turn-around time: usually within 1-2 weeks.

    ● Virus titers provided as at or above stated titer levels.

    ● Production with robust quality control in accordance with NIH Biosafety criteria

    ● Offering small scale pilot production runs, perfect to test infectivity in your chosen cell line.

    ● Flexible virus production scales.

    ● Virus titers determined by qPCR, measuring infectious units per ml (IFUs/ml).

    ● Expert technical assistance provided by Vigene's experienced scientific staff, in most cases response within 12-24 hours, via email.

    ● High efficiency: one of the most efficient ways to deliver cDNA into animal models and hard-to-transfect cell lines.

    ● Good for making stable cell lines.

    Lentiviral Technical Specifications

    ● 100µl of lentivirus > 109 GC/ml or >108 IU/ml enough for 20x10cm cell culture plates See Note

    ● Storage buffer: PBS

    ● Improved biosafety profile with built-in replication incompetent safety

    Lentivirus: most efficient way to deliver cDNA into animal models

    Lentivirus

    The lentivirus is enveloped, spherical to pleomorphic in shape, measuring 80-100 nm in diameter. The mature capsid contains 1572 capsid proteins.

    Lentiviruses are a subset of retroviruses. They are some of the most common and useful viruses used throughout research. They have the ability to transduce both dividing and non-dividing cells without significant immune responses. Integrating stably into the host genome they enable long term transgene expression.

    There are some safety considerations to evaluate when working with lentivirus. The Lentivirus is based on HIV-1, which may require additional lab biosafety procedures. Additionally, this virus integrates randomly into the host genome whereby it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems provide increased researcher safety.

    Lentivirus has broad cell spectrum: infecting most cells, dividing & non-dividing. It is useful for both easy-to-transfect & hard-to-transfect cells. Offering convenience, with minimal need for optimization.

    ● Efficient gene delivery into dividing and non-dividing cells.

    ● Often used in the production of stable cell lines.

    ● Perfect for creating long term modifications, in vivo and in cell uses.

    ● Efficient in transducing T cells, due to their retroviral nature.

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.

    ORF shuttling systems and controls

    Looking for more options in ORF shuttling systems and controls, then please see our corresponding sections:

    Mammalian expression vectors/ORF Shuttling systems

    Control virus options

    Note: The titer of lentivirus is very sensitive to the size of viral genome. Offered titer is based on that the viral genome, insert between two LTRs, is less than 5.1kb. And the gene of interest is less than 1.5kb. Every kb increases of the viral genome, the titer will be decreased by 10 times. Genome copy (GC) is measured by real-time qPCR of lentivirus; infection unit (IU) is measured by fluorescent microcopy of GFP expressing cells.