• Control Viruses & Promoters


    Vigene offers an array of prepackaged & ready-to-use control AAV, lentiviruses, or adenoviruses containing a variety of promoters, fluorescent proteins (FPs), and other reporters.

    Vigene's expansive viral control selection can be used as positive controls for transduction or as negative control in experiments, testing how well AAV, lentivirus, or adenovirus works with your specific cell types.

    New additions to the control viral vector product line include ready-to-use AAV controls with a range of promoters and reporters. Click here to order AAV custom controls or explore our complete AAV control catalogue.

    Which AAV control should I be using?

    GFP-Virus Testing Kit

    Custom AAV Controls, with choice of promoter and reporter ** NEW **

    Popular Pre-made Custom AAV Controls ** NEW **

    Ready-to-Use Standard Controls** NEW **

    Tissue Specific Promoters

    Fluorescent Protein Guide

    Which AAV control should I be using?

    When choosing an AAV control there are three factors you need to take into consideration:

    ● AAV serotype

    ● Promoter

    ● Reporter

    The same serotype and promoter should be used as to that of the AAV that is expressing your GOI/shRNA/sgRNA. For example, if you are using AAV8-CMV-eGFP, you would need a control AAV with serotype 8 and the CMV promoter to drive the fluorescence reporter (eGFP, mCherry, etc).

    When you are using shRNA or miRNA, then a control AAV that is expressing a scrambled shRNA/miRNA; a scrambled sequence is one which targets no known mammalian genes.

    Below, you will find Vigene's most popular AAV control products.

     

    selection of promoters and markers: promoters including ALB, aMHC, c-fos, CAG, CamKIIa, CK0.4, CK1.3, CMV, cTNT, EF1a, EFFS, GFAP, HCRApoE, insulin, MBP, MCK, Mecp2, NSE, PGK, Rep65, Rpe65, SST, Syn, TBG, UBC, and reporters including Cre, DIO, DIO-GFP, DIO-LacZ, DIO-mCherry, DIO-RFP, GFP, gRNA, LacZ, mCherry, RFP, SaCas9, tTA

    Virus Testing Kits

    With Vigene's GFP Virus Testing Kit and AAV Testing Kit customers only need the one kit to speedily and accurately determine the specific efficiency using different cell lines. The GFP Virus Testing Kit comprises of 9 different viruses packaged with CMV-GFP, including adenovirus, lentivirus, and AAV in serotypes 1, 2, 5, 6, 7, 8, and 9. View GFP-testing kit results, HEK293 cells, 48 hours post transfection. The AAV Testing Kit includes CMV-GFP packaged into AAV serotypes 1, 2, 5, 6, 8, 9, and DJ.

    • Cat.#
    • Description
    • Titer (GC/ml)
    • Qty
    • Price
    CT0001 GFP Virus Testing Kit 1x109-13 50µl
    $1,399
    CT0002 AAV Testing Kit 1x1013 50µl
    $1,250

     

    Popular Pre-made AAV Controls

    The most popular controls are listed in the table below. More pre-made AAV controls are available for all tissues and popular tags and markers can be explored from our control catalogue and you may place your order here. Custom orders accepted.

    Promoter

    Marker

    Product Name

    Titer and Volume

    Cat #

    Order

    CamKIIaCreAAV-CamKIIa-CreTiter >1x1013GC/ml, volume 100µl CV-17054 order here
    CamKIIaeGFPAAV-CamKIIa-eGFPTiter >1x1013GC/ml, volume 100µl CV-17002 order here
    CamKIIamCherryAAV-CamKIIa-mCherryTiter >1x1013GC/ml, volume 100µl CV-17031 order here
    CamKIIaRFPAAV-CamKIIa-RFPTiter >1x1013GC/ml, volume 100µl CV-17015 order here
    EF1aGFPAAV-EF1a-GFPTiter >1x1013GC/ml, volume 100µl CV-17171 order here
    GFAPCreAAV-GFAP-CreTiter >1x1013GC/ml, volume 100µl CV-17052 order here
    GFAPCreAAV-GFAP-CreTiter >1x1013GC/ml, volume 100µl CV-17057 order here
    GFAPeGFPAAV-GFAP-eGFPTiter >1x1013GC/ml, volume 100µl CV-17006 order here
    GFAPmCherryAAV-GFAP-mCherryTiter >1x1013GC/ml, volume 100µl CV-17037 order here
    GFAPRFPAAV-GFAP-RFPTiter >1x1013GC/ml, volume 100µl CV-17021 order here
    SynCreAAV-Syn-CreTiter >1x1013GC/ml, volume 100µl CV-17053 order here
    SyneGFPAAV-Syn-eGFPTiter >1x1013GC/ml, volume 100µl CV-17001 order here
    SynmCherryAAV-Syn-mCherryTiter >1x1013GC/ml, volume 100µl CV-17030 order here
    SynRFPAAV-Syn-RFPTiter >1x1013GC/ml, volume 100µl CV-17014 order here

     

    vector backbone of AAV and AAV-DIO vectors

    Ready-to-Use Standard Controls

    GFP Standard Virus Controls

    RFP Standard Virus Controls ** NEW **

    mCherry Standard Virus Controls ** NEW **

    Custom Adenovirus Controls

    AAV, Adenovirus and Lentivirus: eGFP Control with Ubiquitous CMV

    CV10001 Control GFP Adenovirus is the recombinant human adenovirus type 5 which expresses enhanced green fluorescent protein (eGFP) under the control of the CMV promoter. eGFP is a GFP mutant with improved fluorescence and stability. Recombinant eGFP adenovirus serves as a control for other recombinant adenoviruses. CV10001 adenovirus can be used to determine transduction efficiency as well as to optimize viral infection conditions in specific cell types.

    Promoter: CMV

    Volume: 50µl or 100µl

    AAV controls contains both AAV serotype 2 capsid & ITR, and expresses eGFP. The eGFP expression is under the control of a CMV promoter. AAV Vector Backbone Sequence: pAV-FH

    Explore additional control options. Order AAV eGFP below.


    • Cat.#
    • Description
    • Titer
    • Qty
    • Price
    CV10001 Control GFP Adenovirus 1x1012VP/mL 50µl
    $349
    100µl
    $495
    CV10002 Control GFP Lentivirus 1x109IFU/mL 50µl
    $349
    100µl
    $495
    CV10003 Control GFP AAV Serotype 1 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10004 Control GFP AAV Serotype 2 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10005 Control GFP AAV Serotype 5 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10006 Control GFP AAV Serotype 6 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10007 Control GFP AAV Serotype 7 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10008 Control GFP AAV Serotype 8 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10009 Control GFP AAV Serotype 9 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10028 Control GFP AAV Serotype DJ 1x1013GC/mL 50µl
    $349
    100µl
    $495

    AAV and Adenovirus: RFP Control with Ubiquitous CMV

    Promoter: CMV

    Volume: 50µl or 100µl

    AAV controls contain both AAV serotype 2 capsid & ITR, and expresses RFP. The RFP expression is under the control of a CMV promoter. AAV Vector Backbone Sequence: pAV-FH


    • Cat.#
    • Description
    • Titer
    • Qty
    • Price
    CV10011 Control RFP Adenovirus 1x1010VP/mL 50µl
    $349
    100µl
    $495
    CV10014 Control RFP AAV Serotype 1 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10015 Control RFP AAV Serotype 2 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10016 Control RFP AAV Serotype 5 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10017 Control RFP AAV Serotype 6 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10018 Control RFP AAV Serotype 8 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10019 Control RFP AAV Serotype 9 1x1013GC/mL 50µl
    $349
    100µl
    $495

    Return to Top

    AAV: mCherry Control with Ubiquitous CMV

    Promoter: CMV

    Volume: 50µl or 100µl

    AAV controls contain both AAV serotype 2 capsid & ITR, and expresses mCherry. The mCherry expression is under the control of a CMV promoter. AAV Vector Backbone Sequence: pAV-FH


    • Cat.#
    • Description
    • Titer
    • Qty
    • Price
    CV10020 Control mCherry AAV Serotype 1 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10021 Control mCherry AAV Serotype 2 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10022 Control mCherry AAV Serotype 5 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10023 Control mCherry AAV Serotype 6 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10024 Control mCherry AAV Serotype 8 1x1013GC/mL 50µl
    $349
    100µl
    $495
    CV10025 Control mCherry AAV Serotype 9 1x1013GC/mL 50µl
    $349
    100µl
    $495

    Return to Top

    Adenovirus: Custom Controls with Ubiquitous CMV

    Promoter: CMV

    Volume: 50µl or 100µl


    • Cat.#
    • Description
    • Titer
    • Qty
    • Price
    CV10010 Cre-Adenovirus 1x1010VP/mL 50µl
    $349
    100µl
    $495
    CV10012 Luciferase Adenovirus 1x1010VP/mL 50µl
    $349
    100µl
    $495
    CV10013 LacZ/Beta-galactosidase Adenovirus 1x1010VP/mL 50µl
    $349
    100µl
    $495

    Return to Top

    Fluorescent Protein Guide

    Recommendations by Spectral Class

    The table below presents a general recommendation for the most commonly used spectral classes.

    Table. FP by Spectral Class showing excitation/emmission

    Color

    Fluorescent Protein (excitation/emmission, nm)

    Description

    Far-red

    mPlum (590/649), mRaspberry (598/625)

    ▪ Reasonable brightness, a photostable monomer, not as bright as many shorter-wavelength options.

    ▪ Useful when the spectral separation from other FPs is critical.
    ▪ May have some advantage when imaging thicker tissues.

    Red

    mCherry (587/610), mStrawberry (574/596)

    ▪ mCherry is the best general-purpose red monomer with superior photostability.

    ▪ mStrawberry is the brightest, but less photostable than mCherry; should be avoided when photostability is critical.

    Orange

    mOrange (548/562), tdTomato (554/581),

    ▪ Brightest orange monomer.

    ▪ Not ideal when photostability is critical or when targeted to regions of low or unstable pH.

    ▪ tdTomato is equally photostable as mCherry but twice the molecular weight.

    ▪ tdTomato best used when fusion tag size does not interfere with protein function.

    Yellow-Green

    mCitrine (516/529), mVenus (515/528), YPet (517/530)

    ▪ FRET applications: YPet advised to be used in conjunction with the CFP variant CyPet, though it is prone to dimerization.

    ▪ EYFP is obsolete.

    ▪ Members perform well in most applications.

    Green

    eGFP (488/507), mEmerald (487/509)

    ▪ mEmerald, the newer variant exhibits, far more efficient folding at 37 °C than the common variant eGFP

    Cyan

    Cerulean (433/475), CyPet (435/477), mCFP (433/475)

    ▪ Best general purpose CFP is Cerulean, it is the brightest and folds most efficiently at 37 °C.

    ▪ CyPet is a better choice than mCFP; it has a more blue-shifted and narrower emission peak.

    ▪ CyPet displays efficient FRET with YFP variant YPet.

    A Note to Fluorescent Proteins

    Fluorescent proteins are genetically encoded tools that, due to their size and ease of use, have become staple in the molecular biology laboratory. In addition to the ready-to-ship individual GFP control viruses Vigene offers a range of fluorescence protein tags. Please also review the sections on Applications using fluorescent proteins, FP Recommendations by Spectral Class guide, and Explore tissue-specific promoters.

    Key Advantages using Fluorescent Controls and Tags

    ● Quantify transduction efficiency using fluorescence (e.g. GFP)
    ● Optimize transduction conditions in your chosen cell system
    ● Visually confirm successful transduction
    ● Premade & Ready-to-use
    ● Next Day delivery

    Vigene has pioneered the pEnter Entry Vector that is compatible with a variety of destinations vectors. This helps to better accommodate the diverse need of tagging, different tag types and/or different locations - view our vectors.

    If you do not see your choice of fluorescent proteins, please contact us so we can discuss your needs.

     

    Purified AAV - GFP expression in cortical neurons
    Table. Characteristics of most popular fluorescent proteins

    Color

    Protein

    Excitation
    (nm)

    Emission
    (nm)

    Brightness

    Structure

    Green

    EGFP

    488

    507

    34

    Prone to dimerizationSee note

    Red

    mCherry

    587

    610

    16

    Monomer

     

    mRFP1

    584

    607

    13

    Monomer

    Orange

    tdTomato

    554

    581

    95

    Tandem-dimer

    Yellow

    EYFP

    513

    527

    51

    Prone to dimerization

    A note on eGFP dimerization

    EGFP/GFP is prone to forming noncovalent dimers. This can be problematic and can ultimantely lead to significant artifacts. Specifially, this can become a problem when you implement the use of EGFP/GFP for fusion proteins - by fusing GFP to your protein of interest to study the protein's behavior. There is, however, no problem in using EGFP/GFP as a transcriptional reporter or as a general cytoplasmic label of cells. To read more about this topic please refer to a paper by Costantini, L.M., et al., Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035.

    Accomplish High Transfection Efficiency with Adenoviral Transfection

    Image below shows the high transfection efficiency with Vigene's Adenovirus GFP control. Images are 42h post-infections, with Vigene's Adenovirus GFP control, at a titer of 6x109vp/ml, on a 12 well plate (0.75x106cells/well) in hepatocyte cells.


    High transfection efficiency with adenoviral transfection

     

    Return to Top

     

    Applications using fluorescent proteins

    Fusion Tagging

    Fluorescent proteins can be fused to the N- or C-terminus of proteins, allowing visualization of where and when the gene is expressed.

    Biosensors

    GFP-based fluorescent biosensors have been designed to detect a variety of intracellular conditions, such as ion (e.g. Ca2+) concentrations, calmodulin, glutamate and others. Review Vigene's Biosensor AAV collection of biosensors.

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.

    Promoters

    Cell and tissue-specific promoters have specific activity in certain cell types. Vigene offers a selection of cell and tissue-specific promoters to complement your viral vector needs. If you don't see the promoter your interested in using, please contact us for a custom quote.

    Cat.#Promoter
    Name
    SizeTissue or Cell Specificity
    PM10001 ALB 2.4kb Liver
    PM10002 GFAP104 845bp Glial, hybrid of EF1a and GFAP
    PM10003 CAG 944bp Ubiquitous
    PM10004 CaMKIIa 1.2kb Excitatory neurons in the neocortex and hippocampus
    PM10005 EF1a 1.2kb Ubiquitous
    PM10006 CK1.3 1.1kb Excitatory neurons in the neocortex and hippocampus, truncated CaMKIIa
    PM10007 CK0.4 217bp Excitatory neurons in the neocortex and hippocampus, truncated CaMKIIa
    PM10008 GFAP 2.0kb Astrocytes
    PM10009 MBP 1.3kb Oligodendrocytes
    PM10010 EFFS 253bp Ubiquitous, truncated EF1a
    PM10011 TBG 460bp Liver
    PM10012 aMHC 372bp Heart
    PM10013 cTNT 702bp Cardiomyocytes
    PM10014 Synapsin 471bp Neurons
    PM10015 MeCP2 230bp Neurons
    PM10016 c-Fos 1.7kb Neurons
    PM10017 MCK 1.36kb Muscle
    PM10018 UBC 1.14kb Ubiquitous
    PM10019 PGK 399bp Ubiquitous
    PM10020 SST 1.23kb GABAergic Neurons
    PM10021 Rpe65 718bp Retina
    PM10022 PDX1 854bp Pancreas
    PM10023 3Xenhancer McK 728bp Muscle
    PM10024 NSE 1.3kb Neurons.