• Control Viruses & Tissue Promoters


    Vigene offers the most complete array of premade, prepackaged & ready-to-transfect control AAV, lentiviruses or adenoviruses containing a variety of fluorescent proteins (FPs) and tags.

    Vigene's expansive viral control selection can be used as positive controls for transduction or as negative control in experiments, testing how well AAV, lentivirus or adenovirus work with your specific cell types.

    AAV controls, with choice of promoter and reporter ** NEW **

    Which AAV control should I be using? ** NEW **

    The Most Popular Pre-made AAV Controls ** NEW **

    AAV eGFP Standard control

    Adenovirus eGFP Standard control

    Lentivirus eGFP Standard control

    GFP-Virus Testing Kit

    Tissue Specific Promoters

    Fluorscent Protein Guide

    AAV control collection

    New additions to the expansive control viral vector product line include ready-to-ship controls for an extensive range or promoters and reporters. Order Controls or explore control catalogue.

    Which AAV control should I be using?

    When choosing an AAV control there are three factors you need to take into consideration:

    ● (1) the AAV serotype;

    ● (2) the promoter; and

    ● (3) the reporter.

    The same serotype and promoter should be used as to that of the AAV that is expressing your GOI/shRNA/sgRNA. For example, if you are using AAV8-CMV-eGFP, you would need a control AAV with serotype 8 and the CMV promoter to drive the fluorescence reporter (eGFP, mCherry, etc).

    When you are using shRNA or miRNA, then a control AAV that is expressing a scrambled shRNA/miRNA; a scrambled sequence is one which targets no known mammalian genes.

    Below, you will find Vigene's most popular AAV control products.

     

    selection of promoters and markers: promoters including ALB, aMHC, c-fos, CAG, CamKIIa, CK0.4, CK1.3, CMV, cTNT, EF1a, EFFS, GFAP, HCRApoE, insulin, MBP, MCK, Mecp2, NSE, PGK, Rep65, Rpe65, SST, Syn, TBG, UBC, and reporters including Cre, DIO, DIO-GFP, DIO-LacZ, DIO-mCherry, DIO-RFP, GFP, gRNA, LacZ, mCherry, RFP, SaCas9, tTA

    The Most Popular Pre-made AAV Controls

    The most popular controls are listed in the table below. 500 more pre- made AAV controls available for all tissues and popular tags and markers can be explored from our control catalogue and you may place your order here. Custom orders accepted.

    Promoter

    Marker

    Product Name

    Titer and Volume

    Cat #

    Order

    CAGDIOAAV-CAG-DIOTiter >1x1013GC/ml, volume 100µl CV-17073 order here
    CAGDIO-GFPAAV-CAG-DIO-GFPTiter >1x1013GC/ml, volume 100µl CV-17090 order here
    CamKIIaCreAAV-CamKIIa-CreTiter >1x1013GC/ml, volume 100µl CV-17054 order here
    CamKIIaDIOAAV-CamKIIa-DIOTiter >1x1013GC/ml, volume 100µl CV-17060 order here
    CamKIIaeGFPAAV-CamKIIa-eGFPTiter >1x1013GC/ml, volume 100µl CV-17002 order here
    CamKIIamCherryAAV-CamKIIa-mCherryTiter >1x1013GC/ml, volume 100µl CV-17031 order here
    CamKIIaRFPAAV-CamKIIa-RFPTiter >1x1013GC/ml, volume 100µl CV-17015 order here
    CMVgRNAAAV-CMV-gRNATiter >1x1013GC/ml, volume 100µl CV-17280 order here
    cTNTmCherryAAV-cTNT-mCherryTiter >1x1013GC/ml, volume 100µl CV-17036 order here
    EF1aDIOAAV-EF1a-DIOTiter >1x1013GC/ml, volume 100µl CV-17058 order here
    EF1aDIO-GFPAAV-EF1a-DIO-GFPTiter >1x1013GC/ml, volume 100µl CV-17076 order here
    EF1aGFPAAV-EF1a-GFPTiter >1x1013GC/ml, volume 100µl CV-17171 order here
    GFAPCreAAV-GFAP-CreTiter >1x1013GC/ml, volume 100µl CV-17052 order here
    GFAPCreAAV-GFAP-CreTiter >1x1013GC/ml, volume 100µl CV-17057 order here
    GFAPDIOAAV-GFAP-DIOTiter >1x1013GC/ml, volume 100µl CV-17074 order here
    GFAPDIO-GFPAAV-GFAP-DIO-GFPTiter >1x1013GC/ml, volume 100µl CV-17091 order here
    GFAPeGFPAAV-GFAP-eGFPTiter >1x1013GC/ml, volume 100µl CV-17006 order here
    GFAPmCherryAAV-GFAP-mCherryTiter >1x1013GC/ml, volume 100µl CV-17037 order here
    GFAPRFPAAV-GFAP-RFPTiter >1x1013GC/ml, volume 100µl CV-17021 order here
    SynCreAAV-Syn-CreTiter >1x1013GC/ml, volume 100µl CV-17053 order here
    SynDIOAAV-Syn-DIOTiter >1x1013GC/ml, volume 100µl CV-17059 order here
    SynDIO-GFPAAV-Syn-DIO-GFPTiter >1x1013GC/ml, volume 100µl CV-17077 order here
    SyneGFPAAV-Syn-eGFPTiter >1x1013GC/ml, volume 100µl CV-17001 order here
    SynmCherryAAV-Syn-mCherryTiter >1x1013GC/ml, volume 100µl CV-17030 order here
    SynRFPAAV-Syn-RFPTiter >1x1013GC/ml, volume 100µl CV-17014 order here

     

    vector backbone of AAV and AAV-DIO vectors

    GFP-Virus Testing Kit

    Vigene's GFP testing kit comprises of 9 different virus. Customers only need the one kit and will be able do speedily and accurately determine the specific efficiency using different cell lines. No more wasting resources and budgets. View GFP-testing kit results, HEK293 cells, 48 hours post transfection.

    • Cat.#
    • Description
    • Titer (GC/ml)
    • Qty(µl)
    • Price
    CT0001 GFP-Virus Testing Kit 1x109-13 50
    $995

     

    Pre-packaged ready-to-use standard eGFP controls

    AAV, Adenovirus and Lentivirus: eGFP Control with Ubiquitous CMV

    CV10001 Control GFP Adenovirus is the recombinant human adenovirus type 5 which expresses enhanced green fluorescent protein (eGFP) under the control of the CMV promoter. eGFP is a GFP mutant with improved fluorescence and stability. Recombinant eGFP adenovirus serves as a control for other recombinant adenoviruses. CV10001 adenovirus can be used to determine transduction efficiency as well as to optimize viral infection conditions in specific cell types.

    Promoter: CMV

    Volume: 50µl

    AAV controls contains both AAV serotype 2 Capsid & ITR, and expresses eGFP. The eGFP expression is under the control of a CMV promoter. AAV Vector Backbone Sequence: pAV-FH

    Explore additional control options. Order AAV eGFP below.

    eGFP Control with Ubiquitous CMV
    • Cat.#
    • Description
    • Titer
    • Qty(µl)
    • Price
    CV10001 Control GFP Adenovirus 1x1012 50
    $349
    CV10002 Control GFP Lentivirus 1x109 50
    $249
    CV10003 Control GFP AAV Virus Serotype 1 1x1013 50
    $349
    CV10004 Control GFP AAV Virus Serotype 2 1x1013 50
    $349
    CV10005 Control GFP AAV Virus Serotype 5 1x1013 50
    $349
    CV10006 Control GFP AAV Virus Serotype 6 1x1013 50
    $349
    CV10007 Control GFP AAV Virus Serotype 7 1x1013 50
    $349
    CV10008 Control GFP AAV Virus Serotype 8 1x1013 50
    $349
    CV10009 Control GFP AAV Virus Serotype 9 1x1013 50
    $349
    CV10010 Cre-Adenovirus 1x1010 50
    $249
    CV10011 Control RFP Adenovirus 1x1010 50
    $249
    CV10012 Luciferase Adenovirus 1x1010 50
    $249
    CV10013 LacZ/Beta-galactosidase Adenovirus 1x1010 50
    $249

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    Fluorscent Protein Guide

    Recommendations by Spectral Class

    The table below presents a general recommendation for the most commonly used spectral classes.

    Table. FP by Spectral Class showing excitation/emmission

    Color

    Fluorescent Protein (excitation/emmission, nm)

    Description

    Far-red

    mPlum (590/649), mRaspberry (598/625)

    ▪ Reasonable brightness, a photostable monomer, not as bright as many shorter-wavelength options.

    ▪ Useful when the spectral separation from other FPs is critical.
    ▪ May have some advantage when imaging thicker tissues.

    Red

    mCherry (587/610), mStrawberry (574/596)

    ▪ mCherry is the best general-purpose red monomer with superior photostability.

    ▪ mStrawberry is the brightest, but less photostable than mCherry; should be avoided when photostability is critical.

    Orange

    mOrange (548/562), tdTomato (554/581),

    ▪ Brightest orange monomer.

    ▪ Not ideal when photostability is critical or when targeted to regions of low or unstable pH.

    ▪ tdTomato is equally photostable as mCherry but twice the molecular weight.

    ▪ tdTomato best used when fusion tag size does not interfere with protein function.

    Yellow-Green

    mCitrine (516/529), mVenus (515/528), YPet (517/530)

    ▪ FRET applications: YPet advised to be used in conjunction with the CFP variant CyPet, though it is prone to dimerization.

    ▪ EYFP is obsolete.

    ▪ Members perform well in most applications.

    Green

    eGFP (488/507), mEmerald (487/509)

    ▪ mEmerald, the newer variant exhibits, far more efficient folding at 37 °C than the common variant eGFP

    Cyan

    Cerulean (433/475), CyPet (435/477), mCFP (433/475)

    ▪ Best general purpose CFP is Cerulean, it is the brightest and folds most efficiently at 37 °C.

    ▪ CyPet is a better choice than mCFP; it has a more blue-shifted and narrower emission peak.

    ▪ CyPet displays efficient FRET with YFP variant YPet.

    A Note to Fluorescent Proteins

    Fluorescent proteins are genetically encoded tools that, due to their size and ease of use, have become staple in the molecular biology laboratory. In addition to the ready-to-ship individual GFP control viruses Vigene offers a range of fluorescence protein tags. Please also review the sections on Applications using fluorescent proteins, FP Recommendations by Spectral Class guide, and Explore tissue-specific promoters.

    Key Advantages using Fluorescent Controls and Tags

    ● Quantify transduction efficiency using fluorescence (e.g. GFP)
    ● Optimize transduction conditions in your chosen cell system
    ● Visually confirm successful transduction
    ● Premade & Ready-to-use
    ● Next Day delivery

    Vigene has pioneered the pEnter Entry Vector that is compatible with a variety of destinations vectors. This helps to better accommodate the diverse need of tagging, different tag types and/or different locations - view our vectors.

    If you do not see your choice of fluorescent proteins, please contact us so we can discuss your needs.

     

    Purified AAV - GFP expression in cortical neurons
    Table. Characteristics of most popular fluorescent proteins

    Color

    Protein

    Excitation
    (nm)

    Emission
    (nm)

    Brightness

    Structure

    Green

    EGFP

    488

    507

    34

    Prone to dimerizationSee note

    Red

    mCherry

    587

    610

    16

    Monomer

     

    mRFP1

    584

    607

    13

    Monomer

    Orange

    tdTomato

    554

    581

    95

    Tandem-dimer

    Yellow

    EYFP

    513

    527

    51

    Prone to dimerization

    A note on eGFP dimerization

    EGFP/GFP is prone to forming noncovalent dimers. This can be problematic and can ultimantely lead to significant artifacts. Specifially, this can become a problem when you implement the use of EGFP/GFP for fusion proteins - by fusing GFP to your protein of interest to study the protein's behavior. There is, however, no problem in using EGFP/GFP as a transcriptional reporter or as a general cytoplasmic label of cells. To read more about this topic please refer to a paper by Costantini, L.M., et al., Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035.

    Accomplish High Transfection Efficiency with Adenoviral Transfection

    Image below shows the high transfection efficiency with Vigene's Adenovirus GFP control. Images are 42h post-infections, with Vigene's Adenovirus GFP control, at a titer of 6x109vp/ml, on a 12 well plate (0.75x106cells/well) in hepatocyte cells.


    High transfection efficiency with adenoviral transfection

     

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    Applications using fluorescent proteins

    Fusion Tagging

    Fluorescent proteins can be fused to the N- or C-terminus of proteins, allowing visualization of where and when the gene is expressed.

    Biosensors

    GFP-based fluorescent biosensors have been designed to detect a variety of intracellular conditions, such as ion (e.g. Ca2+) concentrations, calmodulin, glutamate and others. Review Vigene's Biosensor AAV collection of biosensors.

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.

    Promoters

    Tissue-specific promoters are promoters that have specific activity in only certain cell types. They control gene expression in a tissue-dependent manner. Vigene offers a selection of tissue-specific promoters to complement our customers viral vector needs. If you don't see the promoter your interested in using, please contact us

    Tissue-specific promoters

    A list of the most commonly used tissue-specific promoters.

    Cat.#Promoter
    Name
    SizeDescription
    PM10001 ALB 2.4kb Liver specific 10 timer stronger than CMV after 10 weeks
    PM10002 GFAP104 845bp Hybrid of EF1a and GFAP
    PM10003 CAG 944bp Strong promoter, ubiquitous expression in vivo
    PM10004 CamKIIa 1.2kb Specific expression in excitatory neurons in the neocortex and hippocampus
    PM10005 EF1A 1.2kb Ubiquitous, weaker than CMV but better for in vivo
    PM10006 CK1.3 1.1kb
    PM10007 CK0.4 217bp Calcium/Calmodulin-dependent kinase II alpha
    PM10008 GFAP 2.0kb Specific in astrocyte
    PM10009 MBP 1.3kb Myelin basic protein promoter, efficient transduction of oligodendrocytes by adeno-associated virus type 8 vectors
    PM10010 EFFS 253bp A short version EF1A
    PM10011 TBG 460bp Homo sapiens serpin peptidase inhibitor, clade A
    PM10012 aMHC 0.4kb Mouse myosin heavy chain alpha promoter
    PM10013 cTNT 702bp Specifically transduce cardiomyocytes
    PM10014 Synapsin 471bp Specific in neuron
    PM10015 Mecp2 230bp Truncated Mcep2 neuron specific
    PM10016 c-fos 1.7kb Activity-dependent promoter
    PM10017 MCK 1.35kb Muscle creatine kinase promoter/enhancer
    PM10018 UBC 1.1kb Ubiquitous, weaker than CMV but better for in vivo
    PM10019 PGK 400bp Ubiquitous, weaker than CMV but better for in vivo
    PM10020 Somatostat 1.2kb Restricting expression to GABAergic neuron
    PM10021 Rpe65 700bp Retinal Pigment epithelium-specific expression in vivo and in vitro
    PM10022 Insulin1 1.0kb Specific in beta- cells of the pancreas
    PM10023 3Xenhancer McK 728bp Much stronger than CMV in muscle, inactive in nonmuscle cell lines and mouse liver
    PM10024 NSE 1.3kb Neuron-specific enolase promoter