• Control Viruses


    Vigene offers the most complete array of premade, prepackaged & ready-to-transfect control AAV, lentiviruses or adenoviruses containing a variety of fluorescent proteins (FPs) and tags.

    Vigene's selection can be used as positive control for transduction or as negative control in your experiments, testing how well AAV, lentivirus or adenovirus works with your specific cell types.

    Fluorescent proteins are genetically encoded tools that, due to their size and ease of use, have become staple in the molecular biology laboratory. In addition to the ready-to-ship individual GFP control viruses Vigene offers a range of fluorescence protein tags. Please also review the sections on Applications using fluorescent proteins, FP Recommendations by Spectral Class guide, and Explore tissue-specific promoters.

    Fluorescent Protein Controls

    GFP-Virus Testing Kit

    GFP Control Viruses

     

    Expansive AAV control collection

    Coming soon

    Additional controls for GFP, RFP, mCherry, with added controls for Cre, DIO, LacZ, tTA, gRNA, SaCas9 with choice of promoter and AAV serotype.

    Key Advantages using Fluorescent Controls and Tags

    ● Quantify transduction efficiency using fluorescence (e.g. GFP)
    ● Optimize transduction conditions in your chosen cell system
    ● Visually confirm successful transduction
    ● Premade & Ready-to-use
    ● Next Day delivery

    Vigene has pioneered the pEnter Entry Vector that is compatible with a variety of destinations vectors. This helps to better accommodate the diverse need of tagging, different tag types and/or different locations - view our vectors.

    If you do not see your choice of fluorescent proteins, please contact us so we can discuss your needs.

     

    Purified AAV - GFP expression in cortical neurons
    Table. Characteristics of most popular fluorescent proteins

    Color

    Protein

    Excitation
    (nm)

    Emission
    (nm)

    Brightness

    Structure

    Green

    EGFP

    488

    507

    34

    Prone to dimerizationSee note

    Red

    mCherry

    587

    610

    16

    Monomer

     

    mRFP1

    584

    607

    13

    Monomer

    Orange

    tdTomato

    554

    581

    95

    Tandem-dimer

    Yellow

    EYFP

    513

    527

    51

    Prone to dimerization

    A note on eGFP dimerization

    EGFP/GFP is prone to forming noncovalent dimers. This can be problematic and can ultimantely lead to significant artifacts. Specifially, this can become a problem when you implement the use of EGFP/GFP for fusion proteins - by fusing GFP to your protein of interest to study the protein's behavior. There is, however, no problem in using EGFP/GFP as a transcriptional reporter or as a general cytoplasmic label of cells. To read more about this topic please refer to a paper by Costantini, L.M., et al., Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035.

    Accomplish High Transfection Efficiency with Adenoviral Transfection

    Image below shows the high transfection efficiency with Vigene's Adenovirus GFP control. Images are 42h post-infections, with Vigene's Adenovirus GFP control, at a titer of 6x109vp/ml, on a 12 well plate (0.75x106cells/well) in hepatocyte cells.


    High transfection efficiency with adenoviral transfection

    GFP-Virus Testing Kit

    Vigene's GFP testing kit comprises of 9 different virus. Customers only need the one kit and will be able do speedily and accurately determine the specific efficiency using different cell lines. No more wasting resources and budgets.

    • Cat.#
    • Description
    • Titer (GC/ml)
    • Qty(µl)
    • Price
    CT0001 GFP-Virus Testing Kit 1x109-13 50
    $995

     

    GFP virus testing kit - HEK293 cells 48h post transfection

     

    Individual GFP Control Viruses

    The original green fluorescent protein (GFP) was cloned back in 1992, and since there have been an explosion in engineering numerous GFP-variants and non-GFP proteins, resulting in a diverse color palette for life scientists.

    Vigene's lentivirus GFP control is purified through sucrose gradient ultracentrifugation. The recommended MOI is 5-10, this is because the titer is based on IFU.

    • Cat.#
    • Description
    • Titer
    • Qty(µl)
    • Price
    CV10001 Control GFP Adenovirus 1x1012 50
    $349
    CV10002 Control GFP Lentivirus 1x109 50
    $249
    CV10003 Control GFP AAV Virus Serotype 1 1x1013 50
    $349
    CV10004 Control GFP AAV Virus Serotype 2 1x1013 50
    $349
    CV10005 Control GFP AAV Virus Serotype 5 1x1013 50
    $349
    CV10006 Control GFP AAV Virus Serotype 6 1x1013 50
    $349
    CV10007 Control GFP AAV Virus Serotype 7 1x1013 50
    $349
    CV10008 Control GFP AAV Virus Serotype 8 1x1013 50
    $349
    CV10009 Control GFP AAV Virus Serotype 9 1x1013 50
    $349
    CV10010 Cre-Adenovirus 1x1010 50
    $249
    CV10011 Control RFP Adenovirus 1x1010 50
    $249
    CV10012 Luciferase Adenovirus 1x1010 50
    $249
    CV10013 LacZ/Beta-galactosidase Adenovirus 1x1010 50
    $249

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    Applications using fluorescent proteins

    Fusion Tagging

    Fluorescent proteins can be fused to the N- or C-terminus of proteins, allowing visualization of where and when the gene is expressed.

    Biosensors

    GFP-based fluorescent biosensors have been designed to detect a variety of intracellular conditions, such as ion (e.g. Ca2+) concentrations, calmodulin, glutamate and others. Review Vigene's Biosensor AAV collection of biosensors.

    FP Recommendations by Spectral Class

    The table below presents a general recommendation for the most commonly used spectral classes.

    Table. FP by Spectral Class showing excitation/emmission

    Color

    Fluorescent Protein (excitation/emmission, nm)

    Description

    Far-red

    mPlum (590/649), mRaspberry (598/625)

    ▪ Reasonable brightness, a photostable monomer, not as bright as many shorter-wavelength options.


    ▪ Useful when the spectral separation from other FPs is critical.
    ▪ May have some advantage when imaging thicker tissues.

    Red

    mCherry (587/610), mStrawberry (574/596)

    ▪ mCherry is the best general-purpose red monomer with superior photostability.


    ▪ mStrawberry is the brightest, but less photostable than mCherry; should be avoided when photostability is critical.

    Orange

    mOrange (548/562), tdTomato (554/581),

    ▪ Brightest orange monomer.

    ▪ Not ideal when photostability is critical or when targeted to regions of low or unstable pH.


    ▪ tdTomato is equally photostable as mCherry but twice the molecular weight.


    ▪ tdTomato best used when fusion tag size does not interfere with protein function.


    Yellow-Green

    mCitrine (516/529), mVenus (515/528), YPet (517/530)

    ▪ FRET applications: YPet advised to be used in conjunction with the CFP variant CyPet, though it is prone to dimerization.


    ▪ EYFP is obsolete.

    ▪ Members perform well in most applications.


    Green

    eGFP (488/507), mEmerald (487/509)

    ▪ mEmerald, the newer variant exhibits, far more efficient folding at 37 °C than the common variant eGFP


    Cyan

    Cerulean (433/475), CyPet (435/477), mCFP (433/475)

    ▪ Best general purpose CFP is Cerulean, it is the brightest and folds most efficiently at 37 °C.


    ▪ CyPet is a better choice than mCFP; it has a more blue-shifted and narrower emission peak.


    ▪ CyPet displays efficient FRET with YFP variant YPet.

     

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