• Control Viruses & Tissue Promoters

    Vigene offers the most complete array of premade, prepackaged & ready-to-transfect control AAV, lentiviruses or adenoviruses containing a variety of fluorescent proteins (FPs) and tags.

    Control viral vectors: AAV, Adenovirus, Lentivirus

    Tiussue Specific Promoters

    Control Viruses

    Vigene's selection can be used as positive control for transduction or as negative control in your experiments, testing how well AAV, lentivirus or adenovirus works with your specific cell types.

    Fluorescent proteins are genetically encoded tools that, due to their size and ease of use, have become staple in the molecular biology laboratory. In addition to the ready-to-ship individual GFP control viruses Vigene offers a range of fluorescence protein tags. Please also review the sections on Applications using fluorescent proteins, FP Recommendations by Spectral Class guide, and Explore tissue-specific promoters.

    Fluorescent Protein Controls

    GFP-Virus Testing Kit

    GFP Control Viruses


    Expansive AAV control collection

    Coming soon

    Additional controls for GFP, RFP, mCherry, with added controls for Cre, DIO, LacZ, tTA, gRNA, SaCas9 with choice of promoter and AAV serotype.

    Key Advantages using Fluorescent Controls and Tags

    ● Quantify transduction efficiency using fluorescence (e.g. GFP)
    ● Optimize transduction conditions in your chosen cell system
    ● Visually confirm successful transduction
    ● Premade & Ready-to-use
    ● Next Day delivery

    Vigene has pioneered the pEnter Entry Vector that is compatible with a variety of destinations vectors. This helps to better accommodate the diverse need of tagging, different tag types and/or different locations - view our vectors.

    If you do not see your choice of fluorescent proteins, please contact us so we can discuss your needs.


    Purified AAV - GFP expression in cortical neurons
    Table. Characteristics of most popular fluorescent proteins












    Prone to dimerizationSee note
























    Prone to dimerization

    A note on eGFP dimerization

    EGFP/GFP is prone to forming noncovalent dimers. This can be problematic and can ultimantely lead to significant artifacts. Specifially, this can become a problem when you implement the use of EGFP/GFP for fusion proteins - by fusing GFP to your protein of interest to study the protein's behavior. There is, however, no problem in using EGFP/GFP as a transcriptional reporter or as a general cytoplasmic label of cells. To read more about this topic please refer to a paper by Costantini, L.M., et al., Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035.

    Accomplish High Transfection Efficiency with Adenoviral Transfection

    Image below shows the high transfection efficiency with Vigene's Adenovirus GFP control. Images are 42h post-infections, with Vigene's Adenovirus GFP control, at a titer of 6x109vp/ml, on a 12 well plate (0.75x106cells/well) in hepatocyte cells.

    High transfection efficiency with adenoviral transfection

    GFP-Virus Testing Kit

    Vigene's GFP testing kit comprises of 9 different virus. Customers only need the one kit and will be able do speedily and accurately determine the specific efficiency using different cell lines. No more wasting resources and budgets.

    • Cat.#
    • Description
    • Titer (GC/ml)
    • Qty(µl)
    • Price
    CT0001 GFP-Virus Testing Kit 1x109-13 50


    GFP virus testing kit - HEK293 cells 48h post transfection


    Individual GFP Control Viruses

    The original green fluorescent protein (GFP) was cloned back in 1992, and since there have been an explosion in engineering numerous GFP-variants and non-GFP proteins, resulting in a diverse color palette for life scientists.

    Vigene's lentivirus GFP control is purified through sucrose gradient ultracentrifugation. The recommended MOI is 5-10, this is because the titer is based on IFU.

    • Cat.#
    • Description
    • Titer
    • Qty(µl)
    • Price
    CV10001 Control GFP Adenovirus 1x1012 50
    CV10002 Control GFP Lentivirus 1x109 50
    CV10003 Control GFP AAV Virus Serotype 1 1x1013 50
    CV10004 Control GFP AAV Virus Serotype 2 1x1013 50
    CV10005 Control GFP AAV Virus Serotype 5 1x1013 50
    CV10006 Control GFP AAV Virus Serotype 6 1x1013 50
    CV10007 Control GFP AAV Virus Serotype 7 1x1013 50
    CV10008 Control GFP AAV Virus Serotype 8 1x1013 50
    CV10009 Control GFP AAV Virus Serotype 9 1x1013 50
    CV10010 Cre-Adenovirus 1x1010 50
    CV10011 Control RFP Adenovirus 1x1010 50
    CV10012 Luciferase Adenovirus 1x1010 50
    CV10013 LacZ/Beta-galactosidase Adenovirus 1x1010 50

    Return to Top


    Applications using fluorescent proteins

    Fusion Tagging

    Fluorescent proteins can be fused to the N- or C-terminus of proteins, allowing visualization of where and when the gene is expressed.


    GFP-based fluorescent biosensors have been designed to detect a variety of intracellular conditions, such as ion (e.g. Ca2+) concentrations, calmodulin, glutamate and others. Review Vigene's Biosensor AAV collection of biosensors.

    FP Recommendations by Spectral Class

    The table below presents a general recommendation for the most commonly used spectral classes.

    Table. FP by Spectral Class showing excitation/emmission


    Fluorescent Protein (excitation/emmission, nm)



    mPlum (590/649), mRaspberry (598/625)

    ▪ Reasonable brightness, a photostable monomer, not as bright as many shorter-wavelength options.

    ▪ Useful when the spectral separation from other FPs is critical.
    ▪ May have some advantage when imaging thicker tissues.


    mCherry (587/610), mStrawberry (574/596)

    ▪ mCherry is the best general-purpose red monomer with superior photostability.

    ▪ mStrawberry is the brightest, but less photostable than mCherry; should be avoided when photostability is critical.


    mOrange (548/562), tdTomato (554/581),

    ▪ Brightest orange monomer.

    ▪ Not ideal when photostability is critical or when targeted to regions of low or unstable pH.

    ▪ tdTomato is equally photostable as mCherry but twice the molecular weight.

    ▪ tdTomato best used when fusion tag size does not interfere with protein function.


    mCitrine (516/529), mVenus (515/528), YPet (517/530)

    ▪ FRET applications: YPet advised to be used in conjunction with the CFP variant CyPet, though it is prone to dimerization.

    ▪ EYFP is obsolete.

    ▪ Members perform well in most applications.


    eGFP (488/507), mEmerald (487/509)

    ▪ mEmerald, the newer variant exhibits, far more efficient folding at 37 °C than the common variant eGFP


    Cerulean (433/475), CyPet (435/477), mCFP (433/475)

    ▪ Best general purpose CFP is Cerulean, it is the brightest and folds most efficiently at 37 °C.

    ▪ CyPet is a better choice than mCFP; it has a more blue-shifted and narrower emission peak.

    ▪ CyPet displays efficient FRET with YFP variant YPet.


    Return to Top



    Tissue-specific promoters are promoters that have specific activity in only certain cell types. They control gene expression in a tissue-dependent manner. Vigene offers a selection of tissue-specific promoters to complement our customers viral vector needs. If you don't see the promoter your interested in using, please contact us

    Tissue-specific promoters

    A list of the most commonly used tissue-specific promoters.

    PM10001 ALB 2.4kb Liver specific 10 timer stronger than CMV after 10 weeks
    PM10002 GFAP104 845bp Hybrid of EF1a and GFAP
    PM10003 CAG 944bp Strong promoter, ubiquitous expression in vivo
    PM10004 CamKIIa 1.2kb Specific expression in excitatory neurons in the neocortex and hippocampus
    PM10005 EF1A 1.2kb Ubiquitous, weaker than CMV but better for in vivo
    PM10006 CK1.3 1.1kb
    PM10007 CK0.4 217bp Calcium/Calmodulin-dependent kinase II alpha
    PM10008 GFAP 2.0kb Specific in astrocyte
    PM10009 MBP 1.3kb Myelin basic protein promoter, efficient transduction of oligodendrocytes by adeno-associated virus type 8 vectors
    PM10010 EFFS 253bp A short version EF1A
    PM10011 TBG 460bp Homo sapiens serpin peptidase inhibitor, clade A
    PM10012 aMHC 0.4kb Mouse myosin heavy chain alpha promoter
    PM10013 cTNT 702bp Specifically transduce cardiomyocytes
    PM10014 Synapsin 471bp Specific in neuron
    PM10015 Mecp2 230bp Truncated Mcep2 neuron specific
    PM10016 c-fos 1.7kb Activity-dependent promoter
    PM10017 MCK 1.35kb Muscle creatine kinase promoter/enhancer
    PM10018 UBC 1.1kb Ubiquitous, weaker than CMV but better for in vivo
    PM10019 PGK 400bp Ubiquitous, weaker than CMV but better for in vivo
    PM10020 Somatostat 1.2kb Restricting expression to GABAergic neuron
    PM10021 Rpe65 700bp Retinal Pigment epithelium-specific expression in vivo and in vitro
    PM10022 Insulin1 1.0kb Specific in beta- cells of the pancreas
    PM10023 3Xenhancer McK 728bp Much stronger than CMV in muscle, inactive in nonmuscle cell lines and mouse liver
    PM10024 NSE 1.3kb Neuron-specific enolase promoter

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.