• AAV Viral Transduction System

    Vigene offers a wide range of rAAV Services and packaging service: ranging from small crude scale to the custom large purified scale. Vigene Biosciences has developed the most robust and professional AAV packaging service, ranging from small crude scale to the custom large purified scale. Provides high-quality and high-titer viral services. Unfamilair with how AAV works, see our section on introduction to AAV.

    Robust AAV production

    AAV packaging and purification

    AAV custom cloning

    Multiple serotypes

    AAV delivery of Cas9 and sgRNA

    Tissue-specific promoters

    Custom projects: full subcloning and plasmid prep services

    Trans-splicing system for large gene delivery

    Key rAAV services

    Adeno-Associated Virus (AAV) Packaging Service

    ● High titer, 10E13 GC/ml $1398

    ● Rapid turn around, 1-2 weeks

    ● Choice of serotype and/or tissue specific promoter

    ● AAV delivery of Cas9 and sgRNA

    Explore packaging options

    Adeno-Associated Virus (AAV) Cloning Service

    Vigene Biosciences has pioneered the pEnter Entry Vector. The pEnter entry vector is compatible with a variety of destinations vectors, including AAV, adenovirus, lentivirus, and shRNA vectors. You may also Download pENTER sequence.

    ● AAV Human cDNA ORF Cloning

    ● AAV shRNA Cloning

    ● FLEX-ON of Cre dependent inducible expression

    ● Trans-splicing system for large gene delivery

    Explore cloning options

    AAV and rAAV

    Adeno-associated virus (AAV) is a small, single-stranded DNA virus. AAV is thought to be non-pathogenic to humans, with replication possible in the presence of a helper virus, such as Adenovirus. They are not permanently integrated into the host genome. These features make AAV a useful tool for gene delivery into a wide variety of cell types and an attractive vector for gene therapy.

    Common use: gene delivery in-vivo because of their mild immunogencity

    The genome of engineered recombinant AAV (rAAV) vectors contain no pathogenic genes. Inverted terminal repeats (ITR) are present making it impossible for viral self-replication. This “gutless” rAAV has been FDA evaluated as a safe vector for use in human gene therapy.

    Please note: that Vigene does not offer wild type AAV viruses or their controls.

    rAAV Products

    Control Viruses
       ▪ Fluorescent proteins: GFP, RFP
       ▪ Cre recombinase
       ▪ Tissue-specific promoters

    Mammalian Expression Vectors
       ▪ Shuttling Vectors
       ▪ shRNA Vectors

    AAV Biosensors and Optogenetic Tools
       ▪ View cellular activity in living tissue
       ▪ Ready-to-use AAV vectors

       ▪ 14 ZKV ORFs as plasmid DNA, AAV, adenovirus
       ▪ Complete ORF Set or single gene

    Additional Viral Resources

    Ordering Instructions: Shipping and payment information
    Product Manuals: How to use your virus
    FAQs : Answers to commonly asked AAV questions
    Publications: showcasing Vigene products and services
    Recipient instructions: information on viral safety and storage


    Return to Top


    AAV Viral Production Overview


    AAV is produced in-house by our scientists. Transfections are conducted using pAV-FH AAV vector, the Ad Helper vector, and a vector encoding the rep and serotype-specific cap. All of our AAV preparations are purified by gradient ultracentrifugation. We concentrate to purity and provide titers that can be used in in vivo studies. Click on the image below to view at higher resolution.

    Titer determination

    The virus titer is determined by viral genome copy number in 1ml sample by real-time quantitative PCR and compared to a standard curve of a plasmid sample of known concentration. Titering is performed on AAV that has been stored at -80C then thawed. Primers targeting the ITR are used and amplicon detection is made with SYBR green. The following data shows the titering results using the AAV-GFP virus.




    Return to Top


    Quality Control


    AAV protein components are VR1 82kDa, VR2 72kDa and VR3 62kDa. We analyze our viral preparations using polyacrylamide gel electrophoresis (PAGE) followed by silver staining. These methods allow us to determine the molecular weight and relative intensity; Good AAV purification should only show three major protein bands by PAGE. The following image shows the protein components in our purified AAV virus.


    Selecting AAV serotypes

    Serotype determines tissue specificity and is classified by the type of capsid structural protein; Serotype 2 (AAV2) is the most studied. AAV2, themost popular serotype, offers a broad range of tissue infectivity for research purposes.

    So far there are 11 AAV serotypes described, they all have different tropism and can infect cells from multiple diverse tissue types. Tissue specificity is determined by the capsid serotype. The selection of the right serotypes is critical for the efficient delivery of gene into the cells or tissues of interest. Vigene offers AAV cloning and packaging in multiple serotypes.

    Table. Availabe AAV serotypes and their tropism

    Tissue Tropism ( indicates recommended application)
    Muscle Liver Lung Brain Retina Pancreas Kidney
    AAV1 Neurons and glial cells
    AAV5 Lung alveolar cells Neurons and glial cells
    AAV7 Neurons
    AAV8 Neurons
    AAV9 Neurons
    AAV-DJ A mix of 8 naturally occurring serotyes.
    Efficient for various types of human tissues and organs.


    Return to Top


    Tissue-specific promoters

    PM10001 ALB 2.4kb Liver specific 10 timer stronger than CMV after 10 weeks
    PM10002 GFAP104 845bp Hybrid of EF1a and GFAP
    PM10003 CAG 944bp Strong promoter, ubiquitous expression in vivo
    PM10004 CamKIIa 1.2kb Specific expression in excitatory neurons in the neocortex and hippocampus
    PM10005 EF1A 1.2kb Ubiquitous, weaker than CMV but better for in vivo
    PM10006 CK1.3 1.1kb
    PM10007 CK0.4 217bp Calcium/Calmodulin-dependent kinase II alpha
    PM10008 GFAP 2.0kb Specific in astrocyte
    PM10009 MBP 1.3kb Myelin basic protein promoter, efficient transduction of oligodendrocytes by adeno-associated virus type 8 vectors
    PM10010 EFFS 253bp A short version EF1A
    PM10011 TBG 460bp Homo sapiens serpin peptidase inhibitor, clade A
    PM10012 aMHC 0.4kb Mouse myosin heavy chain alpha promoter
    PM10013 cTNT 702bp Specifically transduce cardiomyocytes
    PM10014 Synapsin 471bp Specific in neuron
    PM10015 Mecp2 230bp Truncated Mcep2 neuron specific
    PM10016 c-fos 1.7kb Activity-dependent promoter
    PM10017 MCK 1.35kb Muscle creatine kinase promoter/enhancer
    PM10018 UBC 1.1kb Ubiquitous, weaker than CMV but better for in vivo
    PM10019 PGK 400bp Ubiquitous, weaker than CMV but better for in vivo
    PM10020 Somatostat 1.2kb Restricting expression to GABAergic neuron
    PM10021 Rpe65 700bp Retinal Pigment epithelium-specific expression in vivo and in vitro
    PM10022 Insulin1 1.0kb Specific in beta- cells of the pancreas
    PM10023 3Xenhancer McK 728bp Much stronger than CMV in muscle, inactive in nonmuscle cell lines and mouse liver
    PM10024 NSE 1.3kb Neuron-specific enolase promoter

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.