• rAAV Cloning Services


    Vigene offers complete selection of promoters and highest quality rAAV vectors that can be used to express shRNA, human ORF for in vitro and in vivo studies. Through our proprietary technology and innovative AAV vectors, you will get reliable, reproducible, high purity, high titer viral stock every time you order.

    Vigene rAAV Cloning Services Offered

    Basic AAV Human ORF Cloning

    AAV shRNA Cloning Service

    Custom AAV Vector Cloning

    FLEX-ON of Cre dependent inducible expression

    Trans-splicing system for large gene delivery

    Basic AAV Human ORF Cloning

    Vigene's basic human ORF expression vector is designed to drive our collection of 17,000 human ORFs by CMV promoter. Expressed protein carries C' terminal Flag and His tag for detection.

    Please refer to our Full-length cDNA clone page for human ORF selection.

    Custom AAV Vector Cloning

    CMV is a very strong and the most commonly used promoter in driving gene expression in vitro and in vivo. CMV promoter drives ubiquitous gene expression in most tissue and cell types. Due to the methylation/silencing, expression by CMV promoter decreases in vivo after 10 to 20 weeks. For better tissue or cell type specific gene expression and for long term strong and stable expression, many different promoters are offered by Vigene Biosciences.

    Vigene offers the highest quality rAAV vectors and the most complete AAV Expression Systems that can be used to express shRNA, human ORF, CRISPR in vitro and in vivo studies. Through our proprietary technology and innovative AAV vectors, you will get reliable, reproducible, high purity, high titer viral stock every time you order. Working with Vigene, you will save time & increase efficiency at the most cost-effective price.

     

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    AAV Vector Choices

    All vectors ship next day and come as lyophilized DNA, 5 µg

    Learn more about Tissue Specific Promoters


    • Cat.#
    • Vector
    • Selection
    • Promoter
    • Tag
    • Tag Position
    • Price
    P100034 pAV-FH Ampicillin CMV Flag-His ORF
    $249

     

     

    Vigene AAV Services Key Features

    ● All AAV cDNA cloning vectors are compatible with our Entry clones-choose from 17,000 premade human full-length cDNA ORF clones

    ● Production of AAV without helper adenovirus & nonpathogenic with least immune response

    ● Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV7, AAV8 and AAV9) available

    ● Complete vector system for AAV production including helper plasmid and Rep/Cap plasmid

    ● Different promoters (H1 or U6) and reporters (GFP or RFP) available for shRNA AAV cloning

    ● Superior safety features, ITR sequences and rep/cap genes are on separate plasmids

    Vigene provides a full suite of designing, cloning, packaging and generating transduction-ready recombinant AAV particles. Service also includes preparation, extraction, purification and titration.

    We provide the most customizable service; our scientists will work with you step-by-step and learn about your research goal to design and manufacture the most ideal AAV particles for you.

    For more information or any questions, please contact us.

     

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    Additional Viral Resources

    Ordering Instructions: Shipping and payment information
    Product Manuals: How to use your virus
    FAQs : Answers to commonly asked questions
    Publications: showcasing Vigene products and services
    Recipient instructions: information on viral safety and storage


    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.