• Adenoviral Transduction System


    Vigene Biosciences are the adenovirus experts in the industry. Vigene offers a wide range of adenoviral cloning and packaging services: ranging from small crude scale to the custom large purified scale. Vigene Biosciences has developed the most robust and professional AAV packaging service, ranging from small crude scale to the custom large purified scale. Vigene's Adenoviral system guarantees the expression of your gene of interests (GOI): maximum adenovirus production with timely delivery.

    Custom Adenovirus Production Services

    Adenoviral cloning and packaging

    Extensive collection of premade adenoviruses

    Adenovirus and Recombinant Adenovirus

    Wild-type adenovirus, which includes human adenovirus type 5, is associated with various mild disorders, most notably mild to severe respiratory disease in humans.

    Common use: with a large packaging capacity (>8 kb), high titers, and high levels of transgene expression adenovirus is perfect for targeting a wide range of dividing and non-dividing cell types, with almost 100% efficiency. Another important fact is that adenoviruses do not integrate into the host genome. Some disadvantages do exist including transient transgene expression, as well as the viral capsid induced immune response.

    The entire wild-type adenovirus genome is linear, double-stranded DNA of approximately 36Kb. To ensure replication deficiency, the E1 gene (involved in adenoviral replication) is deleted allowing for safe use as a gene delivery tool.

    Adenoviral Cloning and Packaging Services

    Delivery of your primary virus stock within in 2-4 weeks. Offering High viral yield options with competitive pricing. All our products are sequence-verified, with guaranteed GOI expression

    Adenoviral Cloning Service

    Adenoviral Packaging Service
       ▪ Small Scale Crude Packaging Service
       ▪ Large Scale custom Packaging Service

    Premade Adenovirus

    Adenoviral cDNA clones

    Control Viruses
       ▪ Fluorescent proteins: GFP, RFP
       ▪ Cre recombinase
       ▪ Tissue-specific promoters

    Mammalian Expression Vectors
       ▪ Shuttling Vectors
       ▪ shRNA Vectors

    ZIKA ORFs New!
       ▪ 14 ZKV ORFs as plasmid DNA, AAV, adenovirus
       ▪ Complete ORF Set or single gene

    Additional Viral Resources

    Ordering Instructions: Shipping and payment information
    Product Manuals: How to use your virus
    FAQs : Answers to commonly asked AAV questions
    Publications: showcasing Vigene products and services
    Recipient instructions: information on viral safety and storage

     

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    Adenovirus packaging options

    In addition to providing small and large scale packaging options, we offer full subcloning and plasmid prep services prior to viral particle production.

    Small Scale Crude Packaging 500µl of Adenovirus at 1x1010 vp/ml $545
    This service is the preferred option for our customers who need recombinant adenovirus for in vitro studies only; perfect for initial testing in cells. This option offers a low cost amplification solution.
    Large Scale Packaging 500µl of Adenovirus > 1012-13 vp/ml $1,365
    This service meets the needs of our customers who want to use recombinant adenovirus for in vivo studies, or require really high-titered purified viruses.

    Custom Adenovirus Cloning

    Vigene Biosciences has pioneered the pEnter Entry Vector. The pEnter entry vector is compatible with a variety of destinations vectors, including adenoviral, lentiviral, AAV and shRNA vectors. See section on ORF Shuttling Vectors for more details. You may also Download pENTER sequence.

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.

    Want to know more about adenovirus, see our section on Transfection with Adenovirus: Adenovirus Advantages