• Lentiviral Cloning and Production Services


    Lentiviral-viral mediated gene delivery has become one of the most popular gene delivery tools on par with Adenovirus & AAV virus. It has the advantage to be used for either transient or stable expression, and they can infect both replicative and quiescent cells. Vigene provides a complete service from gene cloning into lentiviral vector to lentivirus production. Taking advantage of one of the largest collections of human full-length cDNA clones in the industry, high titer lentivirus can be generated in 2-3 weeks.

    Vigene Lentiviral Cloning Services Offered

    Basic Lentivirus Human ORF Cloning

    Custom Lentiviral Vector Cloning

    Browse Lentiviral Vectors

    Lentivirus shRNA Cloning Service

    Additional Viral Resources

    Basic Lentivirus Human ORF Cloning

    Vigene provides Lenti-ORF clones, plasmids and lentiviral particles from our collection of over 17,000 human ORFs. Download sequence for pLenti-SV-Puro Vector

    Please refer to our Full-length cDNA clone page for human ORF selection.

    Key Advantages

    ● High titer 10E9 IFU/mL lentivirus can be generated in 2-3 weeks.
    ● All 17,000 Entry clones can be shuttled into lentiviral vectors in 2-3 days.
    ● All 1,273 human miRNA clones can be shuttled into the lenti vector for transient or stable expression.
    ● Multiple destination lentiviral vectors are available with different N-terminal and C-terminal fluorescent or affinity tags.
    ● Multiple shRNA lentiviral vectors are available for RNAi studies.
    ● Flexibility: Low or high titer lentiviruses can be provided upon request.
    ● Stringent QC with gene-specific primers and titer titration with Real-time PCR.
    ● Clone any DNA of your interest to one of our lentiviral vectors for virus production.

     

    Custom Lentiviral Vector Cloning

    Our pLenti-SV-Puro vector contains the CMV promoter. CMV is very strong and the most commonly used promoter in driving gene expression in vitro and in vivo. The CMV promoter drives ubiquitous gene expression in most tissue and cell types. Due to the methylation/silencing, expression by CMV promoter decreases in vivo after 10 to 20 weeks. We offer a selection of pLenti vectors. For better tissue or cell type specific gene expression and for long term strong and stable expression, Vigene offers many different promoters.

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.

    Lentiviral Vectors

    All vectors ship next day as lyophilized 5µg DNA


    • Cat.#
    • Vector
    • Selection
    • Promoter
    • Tag
    • Tag Position
    • Price
    P100020 pLent-Flag-His Ampicillin CMV Flag-His ORF
    $249
    P100021 pLent-GFP-CMV Ampicillin CMV Myc-Flag ORF
    $249
    P100022 pLent-Puro-CMC Ampicillin CMV Myc-Flag ORF
    $249
    P100023 pLent-C-GFP Ampicillin CMV GFP ORF
    $249
    P100024 pLent-C-RFP Ampicillin CMV RFP ORF
    $249
    P100025 pLent-N-GFP Ampicillin CMV GFP ORF
    $249
    P100026 pLent-N-RFP Ampicillin CMV RFP ORF
    $249
    P100027 pLent-N-Flag Ampicillin CMV Flag ORF
    $249
    P100028 pLent-N-Myc Ampicillin CMV Myc ORF
    $249
    P100029 pLent-N-HA Ampicillin CMV HA ORF
    $249

    Additional Viral Resources

    Ordering Instructions: Shipping and payment information
    Product Manuals: How to use your virus
    FAQs : Answers to commonly asked questions
    Publications: showcasing Vigene products and services
    Recipient instructions: information on viral safety and storage


    Vigene provides a full suite of designing, cloning, packaging and generating transduction-ready recombinant lentiviral particles. Service also includes preparation, extraction, purification and titration.

    We provide the most customizable service; our scientists will work with you step-by-step and learn about your research goal to design and manufacture the most ideal lentiviral particles for you.

    Ready to order?

    Know what you need? Perhaps, you don't see what you want? Simply request a quote. You may also refer to our guide on placing orders/requesting quotes.